Flow Cytometry Capacity Building Workshop 2016

Organized and Sponsored by the Immunopharmacology Research Laboratory

FACILITATOR: Joyce Ibana, Ph.D.

TRAINER: Kristine-Ann Buela, Ph.D.
Case Western Reserve University Cleveland, Ohio, USA

Flow cytometry is the measurement of the cellular properties of single cell suspensions as they are moving in a fluid stream, facilitated by lasers and antibodies conjugated to fluorochromes, past a stationary set of detectors.

The capability to rapidly quantify multiple parameters and analyze heterogeneous cell populations on a cell-by-cell basis is arguably the most powerful aspect of analytical flow cytometry. This allows flow cytometry to be used for a wide range of applications, which we covered in this hands-on workshop.

Coverage of the Workshop

  1. Immunophenotyping
  2. Cell Cycle Analysis Using Propidium Iodide Staining
  3. Cell Proliferation by CFSE Staining
  4. Apoptosis
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