Flow Cytometry Capacity Building Workshop 2016
Organized and Sponsored by the Immunopharmacology Research Laboratory
FACILITATOR: Joyce Ibana, Ph.D.
TRAINER: Kristine-Ann Buela, Ph.D.
Case Western Reserve University
Cleveland, Ohio, USA
Flow cytometry is the measurement of the cellular properties of single cell suspensions as they are moving in a fluid stream, facilitated by lasers and antibodies conjugated to fluorochromes, past a stationary set of detectors.
The capability to rapidly quantify multiple parameters and analyze heterogeneous cell populations on a cell-by-cell basis is arguably the most powerful aspect of analytical flow cytometry. This allows flow cytometry to be used for a wide range of applications, which we covered in this hands-on workshop.
Coverage of the Workshop
- Immunophenotyping
- Cell Cycle Analysis Using Propidium Iodide Staining
- Cell Proliferation by CFSE Staining
- Apoptosis